Abstract
In this second lecture, the fundamental principles of cellular self-assembly and self-organization are discussed, as well as the sources of material (cells, tissues) needed to produce organoids. How can a biological system self-organize, and what external conditions are required for this to happen ? The importance of the external environment and culture media in these processes is great, and some illustrations are given of the need to calibrate precise culture conditions for each type of organoid. The main recipes for manufacturing these biological objects are presented :
- self-organization ;
- embryonic sheets, embryo development ;
- organoid manufacture, culture media ;
- starting cell types and origins ;
- differentiation protocols ;
- eS cells and iPS cells.
