Cell volume regulation

The 2024 lecture discusses the physical parameters that determine cell volume, taking into account osmotic, mechanical, chemical and metabolic aspects. An important aspect concerns the validity of what biologists call cellular scaling laws, which relate cell volume to the dry mass of the cell or to the volume of cellular compartments (organelles). Particular attention will be paid to the volume of the nucleus, the coupling between cell volume and the cell cycle, and the effect of the cell environment, such as confinement effects.

General references

E. B. Wilson MacMillan, The cell in development and heredity, New York (1925).

Amanda A. Amodeo and Jan M. Skotheim, "Cell-size control", Cold Spring Harb Perspect Biol, 8:a019083 (2016).

Clotilde Cadart, Larisa Venkova, Pierre Recho, Marco Cosentino Lagomarsino and Matthieu Piel, "The physics of cell-size regulation across timescales", Nature Physics, 15, 993-1004 (2019).

Miriam B. Ginzberg, Ran Kafri, Marc Kirschner, "On being the right (cell) size", Science, 348, 1245075 (2015).

Alan R. Kay, Mordecai P., Blaustein J., "Evolution of our understanding of cell volume regulation by the pump-leak mechanism", Gen. Physiol. 151, 407-416, (2019).

Romain Rollin, Jean-François Joanny, Pierre Sens, "Physical basis of the cell size scaling laws", eLife, (2023).

Gabriel E. Neurohr and Angelika Amon, "Relevance and Regulation of Cell Density", Trends in Cell. Biology, 30, 213 (2020).

Gabriel E. Neurohr, Rachel L. Terry, Jette Lengefeld et al. "Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence," Cell, 176, 1083-1097 (2019).

Jie Lin, Ariel Amir, "The Effects of Stochasticity at the Single-Cell Level and Cell Size Control on the Population Growth," Cell Systems, 5, 358-367 (2017).

M. Scott and T. Hwa, "Bacterial growth laws and their applications," Current Opinion in Biotechnology, 22, 559-565 (2011).

Michael Lynch and Georgi K. Marinov, "The bioenergetic costs of a gene", PNAS, 112, 15690-15695 (2011).

Amit Tzur, Ran Kafri, Valerie S. LeBleu, Galit Lahav, Marc W. Kirschner, "Cell Growth and Size Homeostasis in Proliferating Animal Cells", Science, 325 167-171 (2009).

General references

Documents and media

Program

Cell volume, mass and density

Cell Growth under Mechanical Pressure: Effect of Macromolecular Crowding

Osmotic model " pumps and leaks "

Some couplings that define cell size and density distribution

Cell volume and nucleus volume

Using Mathematical Models and Model-Guided Data Analysis to Understand Cellular Decisions to Grow and Progress the Cell Cycle

Fluctuations and volume regulation (1)

Nuclear Organization and Volume Regulation

Fluctuations and volume regulation (2)

Regulation and Relevance of Cytoplasm Density

Metabolism and cell growth

Correlations and Causation in the Bacterial Cell Cycle

See also