Condensats biologiques

Résumé

Le cours donne une introduction aux condensats biologiques, leur rôle et leur fonction. Il présente la description de ces condensats en termes de transition de phase liquide et les limites de cette description.

Références

"Germline P Granules Are Liquid Droplets that Localize by Controlled Dissolution/Condensation", Brangwynne, Clifford P and Eckmann, Christian R and Courson, David S and Rybarska, Agata and Hoege, Carsten and Gharakhani, Jobin and Julicher, Frank and Hyman, Anthony A, Science, 324, 1729--1732 (2009).

"Active Liquid-Like Behavior of Nucleoli Determines their Size and Shape in Xenopus Laevis Oocytes", Brangwynne, Clifford P and Mitchison, Timothy J and Hyman, Anthony A, Proceedings of the National Academy of Sciences, 108, 4334--4339 (2011).

"Salt-Dependent Rheology and Surface Tension of Protein Condensates Using Optical Traps", Jawerth, Louise M and Ijavi, Mahdiye and Ruer, Martine and Saha, Shambaditya and Jahnel, Marcus and Hyman, Anthony A and Julicher, Frank and Fischer-Friedrich, Elisabeth, Physical Review Letters, 121, 258101 (2018).

"Methods for Physical Characterization of Phase-Separated Bodies and Membrane-Less Organelles", Mitrea, Diana M and Chandra, Bappaditya and Ferrolino, Mylene C and Gibbs, Eric B and Tolbert, Michele and White, Michael R and Kriwacki, Richard W, Journal of Molecular Biology, 430, 4773--4805 (2018).

"Measurements of Protein-Protein Interactions by Size Exclusion Chromatography", Bloustine, J and Berejnov, V and Fraden, S, Biophysical Journal, 85, 2619--2623 (2003).

"On the Role of Phase Separation in the Biogenesis of Membraneless Compartments", Musacchio, Andrea,The EMBO Journal, 41, e109952 (2022).

"How Do Protein Domains of Low Sequence Complexity Work?", Kato, Masato and Zhou, Xiaoming and McKnight, Steven L, RNA, 28, 3--15 (2022).

"Evaluating Phase Separation in Live Cells: Diagnosis, Caveats, and Functional Consequences", McSwiggen, David T and Mir, Mustafa and Darzacq, Xavier and Tjian, Robert, Genes and Development, 33, 1619--1634 (2019).

"A Liquid-To-Solid Phase Transition of the Als Protein Fus Accelerated by Disease Mutation", Patel, Avinash and Lee, Hyun O and Jawerth, Louise and Maharana, Shovamayee and Jahnel, Marcus and Hein, Marco Y and Stoynov, Stoyno and Mahamid, Julia and Saha, Shambaditya and Franzmann, Titus M and others, Cell, 162, 1066--1077, (2015).

"Self-Organizing Motors Divide Active Liquid Droplets", Weirich, Kimberly L and Dasbiswas, Kinjal and Witten, Thomas A and Vaikuntanathan, Suriyanarayanan and Gardel, Margaret L, Proceedings of the National Academy of Sciences, 116, 11125--11130 (2019).

"The Structure of Protoplasm", Wilson, Edmund B, Science, 10, 33--45 (1899).